The 102 passengers who sailed on the Mayflower in September 1620 came from all over England (and not all of them were religiously motivated), but the leaders of the separatist movement came from just a handful of farming villages in Lincolnshire, Nottinghamshire and southern Yorkshire, most within walking distance of one another. This is not the touristy, thatched-cottage part of England, but it is beautiful nonetheless, and last spring my wife and I visited to see what we could learn about her ancestors, who in so many ways are forefathers to us all.
The most important of the Pilgrim villages, and probably the epicenter of the whole separatist movement, is Scrooby, in Nottinghamshire, where William Brewster, the local postmaster and later a Pilgrim leader, lived and held clandestine religious services in a large manor house. Scrooby today is a bit of a backwater and most of the house (which is now in private hands) was demolished in 1636.
Comment: My sister and mother are members of the Mayflower society